Presentation Information
[8a-N203-1][JSAP-Optica Joint Symposia Invited Talk] Cryo-optical microscopy with on-stage rapid freezing
〇Katsumasa Fujita1 (1.Univ. Osaka)
Keywords:
cryo-optical microscopy,super resolution microscopy,Raman microscopy
Capturing rapid cellular dynamics with high spatial resolution remains a major challenge, especially for transient states like ion fluxes or molecular conformations. In this talk, I introduce time-deterministic cryo-optical microscopy, a novel technique that enables millisecond-scale cryofixation of live cells directly on the microscope stage. By integrating a rapid cryogen injection system with 10 ms temporal precision, we freeze dynamic events, such as calcium ion waves, at exact timepoints, preserving both molecular distribution and chemical state. This method combines the strengths of live-cell imaging and cryofixation, providing high signal-to-noise ratios, improved quantitation, and compatibility with super-resolution and Raman microscopy. I will show multimodal imaging of subcellular architecture and ion dynamics in cardiomyocytes and HeLa cells using SIM and Raman mapping. This approach establishes a new paradigm for spatiotemporally resolved, multimodal analysis of living systems with unprecedented precision.