[10p-A13-1~11] 3.3 Biomedical optics

4:00 PM - 4:15 PM JST(7:00 AM - 7:15 AM UTC)Presentation by Applicant for JSAP Young Scientists Presentation Award

[10p-A13-8] Region-selective Raman Microspectroscopy for Molecular Spectral Tracking of Multicellular Samples

〇Takeru Sasaki¹, Wataru Yamamoto¹, Miho Mashima¹, Ryosuke Oketani¹, Kotaro Hiramatsu¹ (1.Kyushu Univ.)

Keywords:

Deep Learning,Time-lapse Measurements,High-speed Raman Imaging

In this presentation, we report a region-selective Raman microspectroscopy method that combines deep-learning-based image recognition with local Raman spectroscopy to track molecular spectral changes in individual cells within a multicellular sample. Cell regions are extracted from bright-field images, and only the required regions are selectively measured, enabling efficient molecular spectral tracking. In addition to demonstrating accelerated analysis through bead identification, we performed 20-hour time-lapse measurements of 100 live cells with a temporal resolution of approximately 5 minutes and acquired molecular spectral changes associated with cell death.