Presentation Information
[10p-A33-7]Laser Biospeckle and Biospeckle Optical Coherence Tomography for Investigation of Lentil Seed Germination under Simulated Microgravity
〇(B)Saanvi Sarma Durbhaka1, Raja Uma Maheshwari1, Hirofumi Kadano1,2 (1.Shibaura Inst, 2.Saitama Univ.)
Keywords:
Biophotonics,Laser Biospeckle,OCT
Seed germination is a highly dynamic biological process that can be influenced by altered gravitational conditions. This study investigates the effects of simulated microgravity on lentil (Lens culinaris) seed germination using a combination of laser biospeckle imaging and Optical Coherence Tomography (OCT). Simulated microgravity conditions were generated using a two-dimensional clinostat at different rotational speeds and compared with stationary controls. Biospeckle analysis was employed to evaluate temporal changes in biological activity, while OCT was used to observe internal structural changes during germination. The results revealed differences in activity and developmental patterns between control and clinorotated seeds, particularly during the early stages of germination. These findings demonstrate the potential of combining biospeckle imaging and OCT as complementary, non-destructive techniques for monitoring seed responses to simulated microgravity and may contribute to future studies of plant growth in space environments.