[11p-N302-1~12] 12.7 Biomedical Engineering and Biochips

4:15 PM - 4:30 PM JST(7:15 AM - 7:30 AM UTC)Presentation by Applicant for JSAP Young Scientists Presentation Award

[11p-N302-11] In situ observation of the freezing process of plant tissues with laser-induced crystallization method

〇Norifumi Fujii¹, Sho Inoue¹, Hozumi Takahashi¹, Takahisa Matsuzaki¹, Hiroshi Yoshikawa¹ (1.Grad. Sch. of Eng., Univ. Osaka)

Keywords:

freezing,laser,cell

Ice crystals formed during the freezing of biological materials, including food products, can damage cellular structures and thereby induce quality deterioration. However, because the timing and location of ice crystal nucleation cannot be controlled, detailed observation and evaluation of the early stages of freezing have remained challenging. In this study, we utilized an ultrashort-pulse laser-induced crystallization technique to achieve in situ observation of the freezing process in plant tissues at the single-cell level. These results are expected to contribute to a better understanding of the mechanisms of freeze-induced damage induced by ice crystallization.