Presentation Information
[8a-C214-12]Dissolved Oxygen Detection Using Graphene Biosensors for Measurement of Catalase Activity in Single Cells
〇Keita Terasaki1, Takao Ono1, Yamanishi Junsuke1, Satoyuki Kawano1 (1.UOsaka)
Keywords:
graphene,biosensor,catalase activity
In this study, we developed a measurement method based on a highly sensitive graphene biosensor for evaluating the activity of catalase, an antioxidant enzyme involved in various diseases, at the single-cell level. As a preliminary step toward enzyme activity measurements, dissolved oxygen was successfully detected, and an increase in hole current proportional to the oxygen concentration was observed. In addition, a decrease in current corresponding to oxygen diffusion under a nitrogen atmosphere was measured. Future work will focus on developing a diffusion-based quantitative model to enable the quantification of catalase activity and the assessment of cellular activity.