[8a-C214-1~13] 12.6 Nanobiotechnology

11:00 AM - 11:15 AM JST(2:00 AM - 2:15 AM UTC)Presentation by Applicant for JSAP Young Scientists Presentation Award

[8a-C214-8] Single-Molecule Quantum Discrimination of Vasopressin and Oxytocin for Peptide Hormone Analysis

〇Shinya Sakano¹, Takahito Ohshiro², Yuki Komoto², Wataru Takahagi³, Masateru Taniguchi² (1.Susumu CO., LTD., 2.ISIR-SANKEN, UOsaka, 3.Science Tokyo)

Keywords:

single-molecule detection,Tunnel-Current,Biopolymer

Arginine vasopressin (AVP) and oxytocin (OXT) are structurally similar hypothalamic–pituitary peptide hormones that play central roles in water homeostasis, cardiovascular regulation, social behavior, and emotional control. Because these cyclic nonapeptides differ by only two amino acid residues, their direct and selective identification remains difficult for conventional analytical methods. In this study, we used nanogap-electrode single-molecule quantum sensing to measure tunneling current signals from individual AVP and OXT molecules during translocation through the nanogap. Signal features such as waveform shape, peak current, mean current, and dwell time were analyzed by machine learning to capture single-molecule electrical fingerprints and dynamics. This methodology provides a direct analytical approach for characterizing closely related peptide hormones and their subtle structural differences.