Presentation Information

5:00 PM - 5:15 PM JST(8:00 AM - 8:15 AM UTC)

[8p-C214-10]Sensitive Detection of Fluorescently Labeled Proteins by a Thermoresponsive Hydrogel-Modified Plasmonic Sensor

〇shogo Fujita¹, Dario Cattozzo², Yasunori Nawa¹, Jakub Dostalek², Keiko Tawa¹ (1.Kwansei Gakuin Univ., 2.Czech Academy of Sciences)

Keywords:

plasmon,thermoresponsive hydrogel,fluorescence image

Poly(N-isopropylacrylamide) (pNIPAAm) shows a lower critical solution temperature of approximately 32 °C. It is one of the most widely used thermoresponsive polymers, shrinking above 32 °C and swelling below 32 °C. In this study, we modified the pNIPAAm copolymer on a plasmonic chip surface coated with a silver thin film having a wavelength-scale periodic structure, then chemically conjugated biotin to the surface and investigated the detection limit of Cy5-streptavidin (Cy5-SA) using fluorescence microscopy.

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