Presentation Information
[8p-C214-11]Analysis of Membrane Proteins in Exosomes Secreted by Cultured Breast Cancer Cells of MCF-7 and MDA-MB-231 Using a Plasmonic Chip
〇Rina Nagayama1, Tatsuki Iwamura1, Yasunori Nawa1, Hajime Shigeto2, Shohei Yamamura2, Keiko Tawa1 (1.Kwansei Gakuin Univ., 2.AIST)
Keywords:
Exosomes,Plasmon resonance,Membrane protein
Exosomes (EVs) are extracellular vesicles with a diameter of approximately 100 nm, that mediate intercellular communication. In addition, they are promising biomarkers because they contain miRNAs and various proteins. However, direct observation of single EVs is difficult because their size is below the diffraction limit of optical microscopy. Our laboratory has established a quantitative method to detect single EVs as individual bright spots using a plasmonic chip with a periodic structure covered by a thin metal film that enhances fluorescence signals. In this study, we quantitatively evaluated three types of membrane proteins in EVs derived from two kinds of cultured breast cancer cells using plasmon-enhanced fluorescence imaging.