Presentation Information
[9p-N304-4]Computational 4D imaging for understanding pain-processing neural circuit
〇Takuma Sugi1 (1.Hiroshima Univ.)
Keywords:
Light-field microscopy,Neuroscience
Light-field (LF) imaging, a form of computational imaging that integrates optics and information science, enables single-shot 3D imaging without spatial scanning. We overcame the low-spatial-resolution problem that has hindered the broad adoption of LF imaging and achieved large-scale neuronal population recording at cellular resolution, with ultrahigh speed of 100 volumes per second and an ultrawide field of view of 2.5 mm × 1.5 mm, without the need for two-photon excitation. Using this technology, we discovered that the manifold representing pain stimuli in the brain is a loop-like manifold, and identified a hub-mediated recurrent reconfiguration as its circuit-level emergent mechanism. Furthermore, we developed light-field endoscopic imaging technology, enabling cellular-resolution 4D deep-brain Ca2+ imaging at a temporal resolution of 10 volumes per second. By combining this with temperature measurement using fluorescent nanodiamonds as quantum sensors, we established a technique for measuring deep-brain temperature at cellular resolution and discovered that deep-brain temperature fluctuates by as much as 7.39°C.