[15a-M_110-1~11] 3.3 Biomedical optics

11:00 AM - 11:15 AM JST(2:00 AM - 2:15 AM UTC)

[15a-M_110-8] Longitudinal observation of cardiac looping under ethanol exposure

〇Ryuichiro Yamazaki¹, Rikuto Noguchi¹, Aimi Yamashita¹, Taiyo Eguchi¹, Yuuta Moriyama¹, Toshiyuki Mitsui¹ (1.Aogaku Univ.)

Keywords:

optical coherence tomography,biophysics

We developed a longitudinal imaging platform that integrates shell-less (ex ovo) chick embryo culture with swept-source optical coherence tomography (SS-OCT) to interrogate how acute ethanol exposure during early cardiogenesis perturbs cardiac morphogenesis. Using serial OCT cross-sections acquired over development, ethanol-exposed embryos were stratified into three phenotypic groups based on heart-rate trajectories. Beyond global heart-rate changes, OCT-enabled functional readouts revealed characteristic abnormalities in myocardial coordination, including shortening and even reversal of apparent contraction propagation between cardiac regions, together with reduced luminal contrast in the OCT images. We discuss how these time-resolved structural/functional signatures may relate to mechanisms underlying congenital heart defects.