Presentation Information
[15a-PA4-20]In Vitro Investigation of an Emerging Cold Atmospheric Plasma and 64Cu Combination Therapy for Breast Cancer
〇(P)Mary Mikhail1, Nobuya Hayashi1 (1.IGSES, Kyushu Univ.)
Keywords:
Copper-64,Cold Atmospheric Plasma,Cancer therapy
Despite advances in cancer therapy, current treatments remain limited, underscoring the need for innovative anticancer strategies. Cold atmospheric plasma (CAP) has emerged as a promising non-invasive modality that generates reactive oxygen and nitrogen species capable of selectively affecting cancer cells. Copper-64 (64Cu) is a clinically relevant radioisotope used in both cancer diagnosis and therapy. In this study, we investigated a novel combinatorial strategy integrating CAP and 64Cu to evaluate their synergistic anticancer effects. CAP or low-dose 64Cu treatment alone increased cell proliferation, likely due to sublethal ROS levels. Notably, the combined CAP and 64Cu treatment resulted in a modest increase in proliferation compared with individual treatments, suggesting an interaction between plasma-induced oxidative stress and radiation effects. Although the combined response remained below the cytotoxic threshold, these findings suggest synergistic behavior under the present conditions and highlight the need for further optimization of plasma exposure and radioisotope dose to achieve therapeutic cytotoxicity.