Presentation Information

[15a-S4_202-4]Bacterial Detection Using Optical and Electrochemical Properties of Organic-Inorganic Hybrid Nanoparticles

〇Koki Takenaga1, Shintaro Tsuda1, Satohiro Itagaki1, Yojiro Yamamoto1, Hiroshi Shiigi1 (1.Osaka Metropolitan Univ.)

Keywords:

molecularly imprinted polymer,metal nanoparticle

We developed hybrid nanoparticles composed of a thermoresponsive polymer and metal nanoparticles as labels for bacterial detection. The binding of these labels to the bacteria resulted in a scattering intensity approximately three times higher than that of the bacteria alone. In the electrochemical detection of Escherichia coli O26 using this label, a strong correlation was observed between the bacterial counts on the electrode and the oxidation current at +0.14 V (vs. Ag|AgCl). These results demonstrate that the hybrid nanoparticles function effectively as electrochemical labels, enabling the quantification of E. coli O26.