[15a-WL1_201-1~11] 12.6 Nanobiotechnology

9:15 AM - 9:30 AM JST(12:15 AM - 12:30 AM UTC)

[15a-WL1_201-2] Comparison of ssDNA Translocation Waveforms Using Electroless Gold Plating (ELGP) Nanopores at Room Temperature and Elevated Temperatures

〇ZHENGZE GUO¹, Yoh Onozawa¹, Minoru Yano², Seiichiro Izawa¹, Yohei Sasagawa², Itoshi Nikaido², Yutaka Majima¹ (1.Science Tokyo MSL, 2.Science Tokyo MRL)

Keywords:

Nanopore sequencer

In this study, we compared ssDNA translocation events at room temperature and high temperature (65°C) using Electroless Gold Plating (ELGP) nanopores. The ELGP nanopores operated stably even after 72 days from the start of measurement. Under high-temperature conditions, the translocation speed increased approximately 15-fold compared to room temperature. These results suggest that ELGP nanopores possess high chemical and mechanical stability and are capable of measuring ssDNA translocation events in high-temperature environments.