[15a-WL1_201-1~11] 12.6 Nanobiotechnology

11:00 AM - 11:15 AM JST(2:00 AM - 2:15 AM UTC)Presentation by Applicant for JSAP Young Scientists Presentation AwardEnglish Presentation

[15a-WL1_201-8] Effects of Aqueous Solution Properties on the Detection Performance of a Nanoplastic SPR Biosensor

〇(M2)PRAIRSUNAN CHANPANICH¹, Kimleng Keang¹, Snehal Narayan Wasnik¹, Shuo Cheng¹, Mana Toma¹ (1.Science Tokyo)

Keywords:

nanoplastic,SPR,biosensor

Nanoplastics have become significant environmental concerns to ecosystems and human health. Among them, polystyrene nanoparticles (PS-NPs), a widely used plastic in household and industrial products, are major contributors to nanoplastic pollution. Reliable detection of nanoplastics in environments remains challenging due to small size and aggregation with other contaminants. Here, we developed a surface plasmon resonance (SPR)-based biosensor utilizing a PS-binding peptide. Quantitative SPR analysis showed a detection limit of 1.32 µg/mL for 50nm PS-NPs within 20 minutes in ion-adjusted laboratory water. The application in environmental water was verified by spiking PS-NPs into pond water samples. Additionally, the effects of conductivity, pH, and biomolecule contaminants on detection performance were investigated. Nanoplastic detection with SPR biosensor demonstrates label-free, rapid, and sensitive detection, offering potential for real-world environmental monitoring applications.