[15p-M_110-1~12] 3.3 Biomedical optics

2:00 PM - 2:15 PM JST(5:00 AM - 5:15 AM UTC)

[15p-M_110-1] 3D imaging of biological specimens using the LUCID clearing reagent and the HandySPIM lens-free light-sheet module

Hiroshi Onodera^1,5, Kenji Uehara², Takumi Murakami³, Asami Tanaka¹, Morishita Yusuke⁴, 〇Junji Yumoto^1,4 (1.The Univ. of Tokyo, 2.Miyuki Giken, 3.Nippon Electric Glass, 4.PhotonTech Innovations, 5.Jichi Medical Univ.)

Keywords:

LUCID,HandySPIM,light sheet

This report presents the tissue-clearing reagent LUCID and the LED-based lens-free light-sheet module HandySPIM. Biological specimens cleared with LUCID exhibit high optical transparency and support both fluorescent staining and immunofluorescence staining, enabling multiplex labeling within a single specimen. In addition, cleared specimens can be preserved for more than ten years without degradation. In parallel, HandySPIM generates a light sheet with a thickness of 10–20 µm by simply coupling visible-range LED light (400–600 nm) into a glass sheet of the same thickness, without the use of lenses or other optical components. Furthermore, HandySPIM is equipped with a Z-axis stage. By placing this module on the stage of a conventional two-dimensional microscope, using the emitted light sheet as excitation light, and scanning the cleared specimen along the Z-axis, three-dimensional fluorescence imaging can be achieved. Consequently, the combined use of LUCID and HandySPIM enables simple and low-cost three-dimensional fluorescence imaging of biological specimens.