[15p-M_110-1~12] 3.3 Biomedical optics

2:45 PM - 3:00 PM JST(5:45 AM - 6:00 AM UTC)

[15p-M_110-4] Bond-Selective Fluorescence Detection by Mid-Infrared Generation based on Difference-Frequency Generation of High-Speed Tunable Laser Source

〇Kento Kamei¹, Shun Takahashi¹, Kazuhiro Kuruma², Yasuyuki Ozeki^1,2 (1.EEIS, UTokyo, 2.RCAST, UTokyo)

Keywords:

bond-selective fluorescence microscopy,mid-infrared generation,high-speed multi-color imaging

Bond-selective Fluorescence-detected Infrared-excited (BonFIRE) microscopy which is a combination of bond-selective molecular excitation by mid-infrared light and fluorescence excited by near-infrared light, requires high-speed mid-infrared wavenumber tuning for the rapid acquisition of multiple molecular vibration signatures. We employed a mid-IR generation method using the previously developed high-speed tunable light source and a fan-out periodically-poled lithium niobate crystal. With the generated light, we attempted to detect the BonFIRE signal.