Presentation Information
[16a-PB1-4]Development of a Simultaneous-Detection Super-Resolution Fluorescence Microscope Using a Split Optical System
〇(M1C)Shoma Hiratsuka1, Yuji Tsukamoto1, Ryotaro Ozaki1, Takashi Saitou1 (1.Ehime Univ.)
Keywords:
super-resolution fluorescence microscopy,spatial light modulation
In this presentation, we report the development of a super-resolution fluorescence microscope that simultaneously acquires fluorescence images before and after phase modulation using a split optical system. By combining phase control with a spatial light modulator and image differencing, fast and low-invasive super-resolution imaging based on wide-field acquisition is achieved. Numerical simulations and experimental results using fluorescent beads are presented.