[16p-W2_402-1~17] 12.6 Nanobiotechnology

5:45 PM - 6:00 PM JST(8:45 AM - 9:00 AM UTC)

[16p-W2_402-16] Controlled fusion between supported lipid bilayers and vesicles by pH changes in solution

〇Yurika Inoue¹, Azusa Oshima², Aya Tanaka², Akira Heya¹, Koji Sumitomo¹ (1.Univ. of Hyogo, 2.NTT Basic Res. Labs., BMC)

Keywords:

Supported lipid bilayers,Membrane fusion

We investigated the control of membrane fusion onto supported lipid bilayers (SLBs) by altering the pH in the solution surrounding large unilamellar vesicles (LUVs). SLBs containing acidic phospholipids were formed on a silicon thermal oxide surface coated with the positively charged polymer, poly-L-ornithine. When the positively charged LUVs, generated by acidifying the surrounding solution, were introduced onto the SLBs, the LUVs either adsorbed onto or hemifused with the SLBs. Subsequently, the addition of calcium ions triggered a transition from hemifusion to full fusion. Combining pH modulation with calcium ion addition is expected to enable control of fusion toward the central region of the SLBs.