Presentation Information
[16p-W2_402-7]Gate Interface-Modified Organic Electrochemical Transistors for the Sensitive Detection of Extracellular Electron Transfer in Non-electroactive Bacteria
〇(M2)Zekun Gao1, Shenghan Gu1, Yuhao Xie1, Xenofon Strakosas2, Takeo Miyake1, Saman Azhari1, Gabor Mehes1 (1.Waseda Univ., 2.Linkoping Univ.)
Keywords:
Organic electrochemical transistors,Extracellular electron transfer,Redox polymer
Extracellular electron transfer (EET) enables bacteria to exchange electrons with their environment, underpinning microbial bioelectronic applications. However, non-electroactive bacteria, which dominate natural systems, produce extremely weak EET signals that challenge conventional electrochemical detection. Here, we present an interface-engineered amplification platform for sensitive detection of weak EET.
First, a poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS)/redox polymer (RP) composite layer is constructed on fluorine-doped tin oxide (FTO) substrates as working electrode (WE). Cyclic voltammetry (CV) and chronoamperometry (CA) measurements in microbial electrochemical cells (MEC) demonstrate that RP acts as an efficient electron mediator, significantly enhancing the detectable EET signals of E. coli (Fig. 1a), aligned with our recent work using carbon felt base electrodes1. Building on this mechanism, RP was further integrated onto the gate of an organic electrochemical transistor (OECT) with a PEDOT:PSS channel, enabling glucose-triggered bacterial electron flow to induce ionic dedoping in the channel. This process translates a minute change in gate current (IG) into a pronounced modulation of the drain-source current (IDS), yielding a sensitivity far beyond that of conventional MEC (Fig. 1b). The “RP-mediated EET–OECT amplification” framework offers a strategy for low-power and highly sensitive detection of weak electron outputs from non-electroactive bacteria.
First, a poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS)/redox polymer (RP) composite layer is constructed on fluorine-doped tin oxide (FTO) substrates as working electrode (WE). Cyclic voltammetry (CV) and chronoamperometry (CA) measurements in microbial electrochemical cells (MEC) demonstrate that RP acts as an efficient electron mediator, significantly enhancing the detectable EET signals of E. coli (Fig. 1a), aligned with our recent work using carbon felt base electrodes1. Building on this mechanism, RP was further integrated onto the gate of an organic electrochemical transistor (OECT) with a PEDOT:PSS channel, enabling glucose-triggered bacterial electron flow to induce ionic dedoping in the channel. This process translates a minute change in gate current (IG) into a pronounced modulation of the drain-source current (IDS), yielding a sensitivity far beyond that of conventional MEC (Fig. 1b). The “RP-mediated EET–OECT amplification” framework offers a strategy for low-power and highly sensitive detection of weak electron outputs from non-electroactive bacteria.