Presentation Information
[16p-W2_402-8]Comprehensive Profiling of Biomacromolecular Modifications via Single-Molecule Quantum Sensing
〇Takahito Ohshiro1, Yuki Komoto1, Taniguchi Masateru1 (1.Sanken, Osaka Univ.)
Keywords:
single-molecule detection,Tunnel-Current,Biopolymer
This presentation introduces our latest advances in single-molecule quantum sensing using nanogap electrodes to comprehensively analyze diverse chemical modifications present in biomacromolecules. By measuring quantum tunneling currents generated as individual molecules translocate through a precisely engineered metallic nanogap, we have established an approach capable of distinguishing subtle structural and electronic differences associated with various post-transcriptional and post-translational modifications. This platform enables highly sensitive identification of modifications in RNA, peptides, and larger biomacromolecular assemblies at the single-molecule level. Furthermore, we explore the potential of this technique for expanding epigenomic and epitranscriptomic profiling (“epi-omics”) and for advancing precision medicine, particularly in contexts where low-abundance modified molecules serve as critical diagnostic or functional indicators. Our findings highlight the promise of quantum-scale electrical sensing as a next-generation analytical tool for biological and medical applications.