[17a-WL1_301-1~11] 12.7 Biomedical Engineering and Biochips

11:00 AM - 11:15 AM JST(2:00 AM - 2:15 AM UTC)Presentation by Applicant for JSAP Young Scientists Presentation Award

[17a-WL1_301-8] Equivalent Circuit Analysis of Cellular Senescence Using Electrochemical Impedance Spectroscopy:
Application to a Hydrogen Peroxide–Induced Senescence Model

〇(B)Shuntaro Shibazaki¹, Ippei Yagi¹, Satoshi Uchida¹ (1.Tokyo Metropolitan Univ.)

Keywords:

Senescent cells,Electrochemical impedance spectroscopy,Equivalent circuit fitting

In this study, electrochemical impedance spectroscopy was applied to measure the impedance characteristics of normal and senescent cells using a hydrogen peroxide–induced senescence model, and their electrical properties were analyzed through equivalent circuit fitting. An equivalent circuit model incorporating constant phase elements (CPEs) was employed to account for interfacial non-idealities. The results revealed differences in impedance responses and circuit parameters between normal and senescent cells, particularly in interfacial resistance and capacitive components, suggesting increased interfacial heterogeneity in senescent cells. However, reduced fitting accuracy was observed in low-frequency regions and with prolonged measurement time.