Presentation Information
[17p-PA1-2]Experimental Verification of Spherical Aberration Correction using Modified Pupil Coordinates in High Numerica Aperture Conditions
〇Shunji Kumata1,2, Yuuki Uesugi2, Yuichi Kozawa2 (1.Grad. Sch. of Eng., Tohoku Univ., 2.IMRAM, Tohoku Univ.)
Keywords:
laser microscopy,aberration correction
In laser scanning microscopy using a high numerical aperture (NA) objective lens, spherical aberration caused by refractive-index mismatch in the specimen severely degrades focusing performance. This results in reduced signal intensity and degraded spatial resolution, particularly in deep regions. Here, we report the experimental results of aberration correction performed under conditions of significant refractive index mismatch, using a correction wavefront based on Zernike polynomial expansion with modified pupil coordinates, a method we previously proposed.