Presentation Information
[18a-WL2_201-3]Finite-Element Analysis of Gold Nanoparticle–Induced Intracellular Heating and Experimental Validation by NV-Center Nanodiamond Thermometry
〇(DC)Mina Tavakkoli1, Masazumi Fujiwara1 (1.Okayama University)
Keywords:
nanophotonics,intracellular thermometry,photothermal heating
Real-time intracellular thermometry provides a powerful approach to investigate how localized heating influences subcellular structures and functions. In this study, gold nanoparticles and fluorescent nanodiamonds are employed as intracellular nanoheaters and thermometers to analyze nanoscale heat generation and dissipation inside cells. Finite element simulations indicate that steady-state temperature distributions around gold nanoparticles are mainly determined by the surrounding environment and thermal boundary conditions at the nanoscale. Simulations performed in media with different thermal conductivities demonstrate that local temperature rise is dominated by thermal confinement rather than material properties alone. Guided by these results, preliminary near-infrared laser heating experiments were conducted in HeLa cells, enabling real-time intracellular temperature mapping. The results indicate controllable local temperature elevation within biologically relevant ranges, suggesting the potential of combining gold nanoparticles and nanodiamond thermometry for investigating subcellular thermal responses.