Presentation Information
[XI-20-34]Establishment of an antiviral immunobiotic evaluation system in porcine intestinal epithelial cells and macrophages
*Ryoko Shibata1, Yoshiya Imamura1, Shota Araki1, Sudeb Saha1, Fu Namai1,3, Ayako Miyazaki2, Kohtaro Miyazawa2, Wakako Ikeda-Ohtsubo1,3, Keita Nishiyama1,3, Haruki Kitazawa1,3 (1. Tohoku Univ., 2. NIAH, NARO, 3. CFAI)
[Objective] The present study aimed to investigate the antiviral immunobiotics and their antiviral defense mechanisms in the in vitro co-culture system using porcine intestinal epithelial cells (PIE) and porcine intestinal macrophages (PIM).
[Methods] TEER technique and immunostaining of ZO-1 were used to measure the barrier integrity in PIE. The PIE co-cultured with PIM (PIE co-culture system) and the PIE monoculture were challenged with poly(I:C) to trigger the expression of antiviral related genes, which were measured by RT-qPCR.
[Results] Stimulating the PIE co-culture system with poly(I:C), the expression levels of antiviral related genes were augmented as compared to the PIE monoculture. This result demonstrated the interaction between PIE and PIM cells, suggesting that the system would be valuable to evaluate the antiviral effect of immunobiotics in detail.
[Acknowledgement] BRAIN (No. 01002AB2)
[Methods] TEER technique and immunostaining of ZO-1 were used to measure the barrier integrity in PIE. The PIE co-cultured with PIM (PIE co-culture system) and the PIE monoculture were challenged with poly(I:C) to trigger the expression of antiviral related genes, which were measured by RT-qPCR.
[Results] Stimulating the PIE co-culture system with poly(I:C), the expression levels of antiviral related genes were augmented as compared to the PIE monoculture. This result demonstrated the interaction between PIE and PIM cells, suggesting that the system would be valuable to evaluate the antiviral effect of immunobiotics in detail.
[Acknowledgement] BRAIN (No. 01002AB2)