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Tue. Nov 26, 2024

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Neural mechanism of mouse sexual behavior revealed by the highly-sensitive multiplex fluorescent in situ hybridization

Recent technological innovations in in situ hybridization (ISH) have enabled highly sensitive detection at the single-copy level of mRNA and the simultaneous detection of multiple genes. However, many problems remain to be solved, such as cost barriers and the inability to optimize the signal-to-noise ratio of staining signals due to autofluorescence derived from tissue samples. Dr. Yosuke Tsuneoka, Toho University Faculty of Medicine, has developed a new fluorescent ISH method based on the in situ hybridization chain reaction (isHCR) method. This new isHCR method is a revolutionary ISH method that has many advantages by using short hairpin DNAs. Furthermore, the fluorescence quenching system based on the principle of photobleaching, also developed by Dr. Tsuneoka, can be applied simultaneously to analyze gene expression with higher signal-noise ratio. In this seminar, Dr. Tsuneoka will introduce this new isHCR and present the latest results of his research. Even those who are not interested in gene expression analysis will enjoy this seminar. Please join us!

New frontiers in spatial gene expression analysis with next-generation fluorescence in situ hybridization and autofluorescence quenching

The 47th Annual Meeting of the Molecular Biology Society of Japan

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[2BT-11-01]Neural mechanism of mouse sexual behavior revealed by the highly-sensitive multiplex fluorescent in situ hybridization

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