Session Details
[2BT-11]New frontiers in spatial gene expression analysis with next-generation fluorescence in situ hybridization and autofluorescence quenching
Thu. Nov 28, 2024 11:45 AM - 12:35 PM JST
Thu. Nov 28, 2024 2:45 AM - 3:35 AM UTC
Thu. Nov 28, 2024 2:45 AM - 3:35 AM UTC
Room 11(Fukuoka International Congress Center, 2F 202)
chairperson:Aki Makanae(Nepa Gene Co., Ltd.)
Recent technological innovations in in situ hybridization (ISH) have enabled highly sensitive detection at the single-copy level of mRNA and the simultaneous detection of multiple genes. However, many problems remain to be solved, such as cost barriers and the inability to optimize the signal-to-noise ratio of staining signals due to autofluorescence derived from tissue samples.
Dr. Yosuke Tsuneoka, Toho University Faculty of Medicine, has developed a new fluorescent ISH method based on the in situ hybridization chain reaction (isHCR) method. This new isHCR method is a revolutionary ISH method that has many advantages by using short hairpin DNAs. Furthermore, the fluorescence quenching system based on the principle of photobleaching, also developed by Dr. Tsuneoka, can be applied simultaneously to analyze gene expression with higher signal-noise ratio.
In this seminar, Dr. Tsuneoka will introduce this new isHCR and present the latest results of his research.
Even those who are not interested in gene expression analysis will enjoy this seminar. Please join us!
Dr. Yosuke Tsuneoka, Toho University Faculty of Medicine, has developed a new fluorescent ISH method based on the in situ hybridization chain reaction (isHCR) method. This new isHCR method is a revolutionary ISH method that has many advantages by using short hairpin DNAs. Furthermore, the fluorescence quenching system based on the principle of photobleaching, also developed by Dr. Tsuneoka, can be applied simultaneously to analyze gene expression with higher signal-noise ratio.
In this seminar, Dr. Tsuneoka will introduce this new isHCR and present the latest results of his research.
Even those who are not interested in gene expression analysis will enjoy this seminar. Please join us!
[2BT-11-01]Neural mechanism of mouse sexual behavior revealed by the highly-sensitive multiplex fluorescent in situ hybridization
〇Yousuke Tsuneoka1 (1.Faculty of Medicine, Toho University)