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Wed. Dec 3, 2025

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Expanding the Possibilities of NGS Library Preparation from Single-Stranded DNA

In next-generation sequencing (NGS) analysis, highly fragmented samples such as FFPE-derived DNA and ultra-short DNA fragments have posed significant challenges for conventional library preparation methods.<br>Takara Bio has long provided innovative solutions for low-input DNA analysis through technologies such as ThruPLEX and PicoPLEX. Building on this expertise, we have recently launched the ssDNA-Seq Kit, which incorporates a novel Single-Strand DNA Ligase capable of highly efficient ligation of single-stranded DNA molecules.<br>This kit enables simple and efficient NGS library preparation from both single-stranded and double-stranded DNA.<br>In this seminar, we will introduce the underlying principles and key features of this technology, along with application examples using challenging samples such as FFPE-derived DNA and cfDNA, which have traditionally been difficult to analyze with conventional methods.

Takara bio inc.<br>Expanding the Possibilities of NGS Library Preparation from Single-Stranded DNA

The 48th Annual Meeting of the Molecular Biology Society of Japan

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[ES-03]Expanding the Possibilities of NGS Library Preparation from Single-Stranded DNA

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