The 48th Annual Meeting of the Molecular Biology Society of Japan

Chairperson：Masakazu Ozaki (Agilent Technologies)

High-Quality Proteome Analysis Starting with Sample QC by ProteoAnalyzer

Variability in sample quality is a critical factor that greatly influences the reproducibility of proteome analysis. In particular, for clinical specimens and other valuable research materials, even subtle differences introduced during sample preparation or measurement can significantly affect the final analytical outcome. However, conventional sample QC in proteome analysis has largely been limited to confirming the total protein amount through protein quantification. In this seminar, we will highlight the advantages of an analysis workflow that begins with sample QC using the ProteoAnalyzer, which not only provides molecular weight profiling of proteins similar to SDS-PAGE but also enables absolute quantification based on signal intensity. In addition, we will present our highly reliable proteome analysis approaches that are built upon this QC approach.