講演情報
[2PA-47]Creation of a cyclized cytochrome c555 trimer for protein assembly construction
*Gissi Novientri1, Kodai Fujiwara1, Tsuyoshi Mashima1, Naoya Kobayashi1, Shun Hirota1 (1. Graduate School of Science and Technology, Nara Institute of Science and Technology)
We have previously constructed a building block protein, CPC, based on circular permutation of cytochrome c555 and α-helix insertion. CPC formed a domain-swapped cyclic trimer with open terminal regions, which caused instability in the trimeric structure. Thus, we have covalently connected the terminal regions of the trimer. CPC was mutated to contain the recognition motifs of sortase A, which ligates peptides containing LPETG at the C-terminal and two glycine residues at the N-terminal. The cyclized CPC obtained using sortase A was more thermostable than the CPC trimer. This stable symmetric protein may be useful for constructing protein assemblies.