講演情報
[XI-20-35]Isolation of immunobiotic candidates from maternal microbiota focusing on endogenous antibiotic factors.
*佐久間 大河1、扇 隆介1、櫻井 美月1、中野 祐香1、Saha Sudeb1、生井 楓1,2、周 冰卉1,2、大坪 和香子1,2、須田 義人3、西山 啓太1,2、北澤 春樹1,2 (1. 東北大院農、2. 食と農免疫国際教育研究セ、3. 宮城大食産業)
[Objective]Maternal microbiota has been considered to play a key role in neonatal immune function, but the relevance of these findings to the immune health of piglets is largely unknown. This study aimed to search for immunobiotics that can enhance host defense as alternative to antibiotics, with lactobacilli isolated from maternal microbiota.
[Methods]The microbiome composition in breast milk, vaginal mucus, and feces was analyzed by 16S rRNA gene analysis. Lactobacilli was isolated from these samples, and the species were identified based on their 16S rRNA sequences. Gene expressions of innate immune factors in porcine intestinal epithelial cell stimulated by the isolates were investigated using RT-qPCR.
[Results]L. salivarius, L. helveticus, and L. reuteri were the dominant lactobacilli in milk and vaginal mucus. Several isolates had an ability to modulate NLRs and PGRPs gene expression. These results suggest that isolates from the maternal microbiota are capable of potentially modulating the neonatal innate immunity.
[Acknowledgement]JRA, BRAIN 01002AB2, KAKENHI
[Methods]The microbiome composition in breast milk, vaginal mucus, and feces was analyzed by 16S rRNA gene analysis. Lactobacilli was isolated from these samples, and the species were identified based on their 16S rRNA sequences. Gene expressions of innate immune factors in porcine intestinal epithelial cell stimulated by the isolates were investigated using RT-qPCR.
[Results]L. salivarius, L. helveticus, and L. reuteri were the dominant lactobacilli in milk and vaginal mucus. Several isolates had an ability to modulate NLRs and PGRPs gene expression. These results suggest that isolates from the maternal microbiota are capable of potentially modulating the neonatal innate immunity.
[Acknowledgement]JRA, BRAIN 01002AB2, KAKENHI