Presentation Information
[POS-55]A Non-Invasive, Simple, and Accurate Image Analysis Method for Measuring the Volume of Living Organisms
*Maki Azuma1 (1. Tokyo Metropolitan Musashi Senior High School (Japan))
Keywords:
Image Analysis,Quantification of Regeneration,Volume Measurement,drug screening,Non-Invasive
In recent years, as research on regeneration has become more active, various drugs and methods have been developed to promote regeneration. However, compared to previous qualitative studies, methods for measuring tissue regeneration speed remain limited, and the development of quantitative techniques suitable for high-throughput drug screening has lagged behind. To address this, we investigated a minimally invasive, low-cost, easy-to-use, and general-purpose method for quantifying regeneration, aimed at drug screening applications. Based on previous studies, accurately quantifying tissue growth per unit time is essential for defining regeneration speed. Therefore, we devised a method to measure the volume of living organisms using image analysis based on Pappus's centroid theorem. As a result, in experiments using models, this method enabled volume measurements with 95% confidence. Furthermore, by analyzing data from both direct measurements and image-based calculations, we derived a formula to estimate the true volume. We would like to discuss these latest findings at the conference.