Presentation Information

[SS02-04]Influence of mechanical perturbations due to actions of subnuclear molecules on chromatin organization and dynamics

*Tetsuya Hiraiwa1,7, Rakesh Das2, Takahiro Sakaue3, GV Shivashankar4,5, Jacques Prost6,7 (1. Institue of Physics, Academia Sinica (Taiwan), 2. Max-Planck Institute for the Physics of Complex Systems (Germany), 3. Aoyama Gakuin University (Japan), 4. ETH Zurich (Switzerland), 5. Paul Scherrer Institute (Switzerland), 6. Institut Curie, Paris Science et Lettres Research University (France), 7. Mechanobiology Institute, National University of Singapore (Singapore))

Keywords:

Chromain organization and dynamics,Numerical simulation,Polymer physics

Genetic information in a eukaryotic cell is stored in its chromatin, a composite of DNA and proteins, densely packed within the nucleus. Physical spacing of chromatin is critical in regulating bio-chemical and transcriptional abilities of genes, and proper functionality of the genomic content depends on the nonrandom organization of chromatin. Meanwhile, in a living cell, other subnuclear molecules, like polymerase and topoisomerase, act to facilitate cellular functions. Mechanical perturbation due to such actions of molecules may affect the chromatin organization and dynamics. In this talk, I would like to explain our numerical-simulation studies about such effects, based on polymer-physics concepts and the GPU-aided computations, where we focused on a type of actions of molecules that we call catch-and-release action and implemented in the way inspired by a class of molecules like topoisomerase-II. I will share the results of our simulations on how it affects chromatin organization and dynamics. The results clarified (i) that the mechanical perturbation of such actions can modulate the phase separation organizations of chromatin called heterochromatic and euchromatic regions [1], and (ii) that the mechanical perturbation enhances fluctuating dynamics of inclusions in chromatin through the newly-proposed dynamic mode of chromatin remodeling [2]. --- References: [1] R Das, T Sakaue, GV Shivashankar, J Prost, T Hiraiwa (2022) "How enzymatic activity is involved in chromatin organization", eLife 11, e79901. [2] R Das, T Sakaue, GV Shivashankar, J Prost, T Hiraiwa (2024) “Chromatin Remodeling Due to Transient-Link-and-Pass Activity Enhances Subnuclear Dynamics", Physical Review Letters 132, 058401.