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Mon. Mar 17, 2025

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High-speed fluorescence lifetime imaging microscopy discovers intracellular thermal signaling mechanisms

Fluorescence lifetime is one of the same physical parameter as excitation and emission spectra and provides different information from the intensity images.  The Fluorescence Lifetime Imaging Microscopy (FLIM) using STELLARIS confocal microscopy enables more quantitative measurement of FRET efficiency, biosensors such as calcium ion, separation of fluorophores or fluorophore and autofluorescence and improves the resolution of STED super-resolution microscopy. In this seminar, we will invite Dr. Kohki Okabe (Graduate School of Pharmaceutical Sciences, The University of Tokyo) to present a newly developed high-speed, high-sensitivity imaging technology that enables temperature mapping with higher stability than conventional fluorescence intensity measurements, independent of dye degradation and cell movement. Additionally, Leica will introduce the usefulness of fluorescence lifetime imaging using

Fluorescence lifetime utility: Fluorophore separation, more quantitative FRET, biosensors and STED super-resolution microscopy

APPW2025

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[2LS09-01]High-speed fluorescence lifetime imaging microscopy discovers intracellular thermal signaling mechanisms

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