APPW2025
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Co-sponsored Seminar

[2LS09]Fluorescence lifetime utility: Fluorophore separation, more quantitative FRET, biosensors and STED super-resolution microscopy

Tue. Mar 18, 2025 12:40 PM - 1:30 PM JST
Tue. Mar 18, 2025 3:40 AM - 4:30 AM UTC
Room 9
Chairperson：Shintaro Tanaka（Leica Microsystems K.K.）
Co-hosted by: Leica Microsystems K.K.
Fluorescence lifetime is one of the same physical parameter as excitation and emission spectra and provides different information from the intensity images.  The Fluorescence Lifetime Imaging Microscopy (FLIM) using STELLARIS confocal microscopy enables more quantitative measurement of FRET efficiency, biosensors such as calcium ion, separation of fluorophores or fluorophore and autofluorescence and improves the resolution of STED super-resolution microscopy. In this seminar, we will invite Dr. Kohki Okabe (Graduate School of Pharmaceutical Sciences, The University of Tokyo) to present a newly developed high-speed, high-sensitivity imaging technology that enables temperature mapping with higher stability than conventional fluorescence intensity measurements, independent of dye degradation and cell movement. Additionally, Leica will introduce the usefulness of fluorescence lifetime imaging using
12:42 PM - 1:12 PM JST(3:42 AM - 4:12 AM UTC)

[2LS09-01]High-speed fluorescence lifetime imaging microscopy discovers intracellular thermal signaling mechanisms

KOHKI OKABE (Graduate School of Pharmaceutical Sciences, The University of Tokyo)
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1:12 PM - 1:30 PM JST(4:12 AM - 4:30 AM UTC)

[2LS09-02]Fluorescence lifetime utility: Fluorophore separation, more quantitative FRET, biosensors and STED super-resolution microscopy

Suguru Osari (Leica Microsystems K.K.)
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