The 8th Annual Meeting of The eDNA Society

Contributed Session

December 11 2025 (Thu), 15:30 ~ 17:30, Main Hall
Contributed Session1:

Environmental DNA: Frontiers of On-site Implementation

Organizer:
Yuki Taruma (PWRI, KenKan Consultants Co., Ltd.), Nobutaka Maki (Pacific Consultants Co., Ltd.,), Seiji Sawaki (CTI Engineering Co., Ltd.), Mika Gunji (Nippon Koei Co., Ltd.), Keiko Muraoka (PWRI)

　Environmental DNA (eDNA) technology has recently gained widespread attention as a tool for monitoring aquatic ecosystems and assessing biodiversity, with practical applications advancing across various regions.
　The Ministry of Land, Infrastructure, Transport and Tourism has been collecting fundamental information on the natural environment for over 30 years through the National Census on River Environments (hereafter referred to as the River Census) to appropriately promote the maintenance and conservation of river environments. From FY2026 onward, eDNA surveys will be newly introduced into the River Census, enabling more detailed assessments of river ecosystems. This session will present an overview of the nationwide River Census eDNA survey—what it entails and what types of data will be collected—and discuss potential directions for data utilization. In addition, through examples of eDNA applications across diverse fields, including riverine and terrestrial environments, this session will highlight the frontiers of eDNA implementation through collaboration among industry, government, and academia. 

Host : Nobutaka Maki  (Pacific Consultants Co., Ltd.)

◎Title and Presenter

  • Concept of the session
     Yuki Taruma1,2)
     1) PWRI,  2) KenKan Consultants Co., Ltd.

  • Enhancing the National Census on River Environments through the Introduction of Environmental DNA (eDNA)
     Mai Tsuruta1) 
     1) Ministry of Land Infrastructure Transport and Tourism

  • Environmental DNA Survey Method for River Waterfront Census
     Keiko Muraoka1)
     1) PWRI

  • Issues in the Selection of Sampling Locations and During Sampling
     Akihiro Umemoto1)
     1) FUKKEN CO.,LTD

  • Archiving environmental DNA Samples Remaining After Analysis and Their Future Use
     Takayuki Tanaka1)
     1) PWRI

  • Creating a Potential Distribution Map of Fish across an Entire River Using eDNA Data
    Kento Eguchi1), Shigenari Miyawaki1), Ryota Yokoyama1), Kazuki Kanno1) , Takahiro Fijisawa1), Shinya Takaki1), Ryohei Nakao2), Yoshihisa Akamatsu2), Ryutei Inui3), Norio Onikura4)
     1) KenKan Consultants Co., Ltd., 2) Yamaguchi University, 3) Fukuoka Institute of Technology, 4) Kyushu University

  • Annual Dynamics of Fish Communities Detected through Quantitative eDNA Metabarcoding
    Takeshi Watanabe1), Nanae Nomura1), Taichi Jibiki1), Kazuaki Ohara2), Akiho Yamanaka2), Natsuki Watanabe2)
     1) Pacific Consultants Co., Ltd., 2) MLIT

  • An attempt to assess terrestrial vertebrate fauna through environmental DNA analysis of terrestrial samples
    Hirokazu Hori1), Maki Oosuga1), Seiji Sawaki1), Yuka Munakata2) , Tomomi Yamazaki2)
     1) CTI Engineering Co., Ltd., 2) ER&S co., Ltd.

  • General Discussion
    Moderator : Seiji Sawaki (CTI Engineering Co., Ltd.)
    Commentator : Toshifumi Minamoto (Kobe University)

 

December 12 2025 (Fri), 14:15 ~ 16:15, Main Hall
Contributed Session2:

'False negatives' a major challenge to the practical application of environmental DNA

Organizer:
Yuta Hasebe (Kanagawa Environmental Research Center), Kenji Tsuri (KenKan Consultants Co., Ltd.), Yuka Munakata (Environmental Research & Solutions CO., LTD.), Masaki Takenaka (University of Tsukuba), Hiroto Udagawa (TOKYU CONSTRUCTION CO., LTD.)

Environmental DNA technology has developed rapidly and is being put to practical use with increasing interest in biodiversity conservation. On the other hand, basic research and DNA databases supporting accuracy have not kept pace, which undermines the reliability of surveys. In particular, 'false negatives', in which DNA of organisms that should be present is not detected, can seriously affect the interpretation of results and is a major challenge for practical application. In this free meeting, we would like to introduce examples of false negatives reported in the field and discuss how environmental DNA surveys can be made more reliable.

Host : Yuta Hasebe (KERC)

◎Title and Presenter

  • Approaches to Mitigate PCR Inhibition and Reduce False Negatives in Environmental DNA Metabarcoding
    Kenji Tsuri1,2), Keiko Muraoka2), Keita Hattori2), Takayuki Tanaka2)
     1) KenKan Consultants Co., Ltd., 2) PWRI

  • Case Study: Influence of 2nd PCR Enzyme Selection on Metabarcoding Analysis
     Yuka Munakata1)、Eri Okada1)、Tomomi Yamazaki1)、Takafumi Mizuno1)、Yuta Hasebe2)
     1) Environmental Research & Solutions CO., LTD., 2) Kanagawa Environmental Research Center

  • Proposal for molecular identification using phylogenetic analysis in DNA metabarcoding analysis.
     Masaki Takenaka1)
     1) University of Tsukuba

  • Confronting False Negatives: A User-Side Overview of Operational Issues
     Hiroto Udagawa1)
     1) TOKYU CONSTRUCTION CO., LTD.

  • General Discussion Commentators
     Keiko Muraoka1)
    1) PWRI
◆Note
  •  Most presenters are assumed to use Japanese in the oral sessions, although speakers are encouraged to include English on their slides. 
  • Lectures will be streamed to the session participants via Zoom meeting so that they can apply the translated caption function to the talk. Participants can connect to the Zoom meeting via the venue's Wi-Fi.