Presentation Information
[2SL05-01]Autophagy measurement in cultured cells and mice
Noboru Mizushima (Professor, The University of Tokyo)
Professor, The University of Tokyo
Measuring autophagic activity is crucial for studying its mechanisms, physiological significance, and pathological roles. For this purpose, merely counting the number of autophagosomes or quantifying the LC3 level is insufficient; it is necessary to measure "autophagic flux." In this seminar, I will provide an overview of the recommended biochemical and morphological methods for quantifying autophagic flux in both cultured cells and mice.
Measuring autophagic activity is crucial for studying its mechanisms, physiological significance, and pathological roles. For this purpose, merely counting the number of autophagosomes or quantifying the LC3 level is insufficient; it is necessary to measure "autophagic flux." In this seminar, I will provide an overview of the recommended biochemical and morphological methods for quantifying autophagic flux in both cultured cells and mice.
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