[20a-D901-1~11] CS.14 Code-sharing Session of 12.6 & 12.7

9:00 AM - 9:15 AM JST(12:00 AM - 12:15 AM UTC)

[20a-D901-1] Candida Mannan in saliva detected by plasmon-field enhanced fluorescence imaging

〇Ryuto Noumi¹, Masaya Yako¹, Yasunori Nawa¹, Keiko Tawa¹, Hiroshi Kurita² (1.Kwansei Gakuin Univ., 2.ShinShu Univ.)

Keywords:

plasmon,Bio-imaging,immunosensor

Quantitative evaluation of immunity is useful for health management. Kurita et al. of Shinshu University have shown that Candida mannan (CM) included in oral Candida is an effective marker of immunity. In this study, a saliva sample was measured using a fluorescence sandwich CM immunoassay constructed on a plasmonic chip, and CM was evaluated from fluorescence images with an upright fluorescence microscope. The fluorescence intensity inside the plasmonic pattern was 9.7 ± 2.5 times larger than that outside, and the correlation between fluorescence intensity and CM concentration was shown.