[20a-D901-1~11] CS.14 Code-sharing Session of 12.6 & 12.7

9:15 AM - 9:30 AM JST(12:15 AM - 12:30 AM UTC)

[20a-D901-2] Post-labeling fluorescence microscopy of single exosomes captured by a plasmonic chip

〇Makoto Tokami¹, Yasunori Nawa¹, Keiko Tawa¹ (1.Kwansei Gakuin Univ.)

Keywords:

Plasmon,Single Exosome,Fluorescence microscopy

Exosomes (Extracellular vesicles: Evs) included in the body fluids work as a communication tool between cells and can predict risk affected by a disease in future. Small Evs with φ100 nm are difficult to be observed with conventional optical microscopy. Therefore, in our laboratory, fluorescently labeled Evs (pre-labeling) was observed as bright spots with a plasmon-field enhanced fluorescence microscopy. In this study, fluorescence-labeled antibody was added to Evs captured with a plasmonic chip (post-labeling) and single Evs were observed under the fluorescence microscope.