Presentation Information
[20p-A305-15]Measurement of trans-fat lipolysis in adipose-like cells by multiplex coherent anti-Stokes Raman scattering hyper-spectral imaging
〇(D)Soichiro Homma1, Naoki Yamato2, Mamoru Hashimoto1 (1.Hokkaido Univ. IST, 2.Hokkaido Univ. MDSC)
Keywords:
non-linear Raman scattering microspectroscopy
It has been reported that trans fatty acids can be metabolized differently compared to cis fatty acids, however, the degradation of trans-fats in cells has not been directly observed.
The authors developed the elliptical spot-excitation multiplex CARS (coherent anti-Stokes Raman scattering) spectroscopy microscope capable of high-speed spectroscopic imaging and applied it to the observation of trans fat metabolism to observe fat lipolysis by spatial resolution at the cellular level.
Samples were 3T3L1 cells differentiated into adipose-like cells and fed with the elaidic acid. We measured fat lipolysis with isoproterenol supplemented medium by time-lapse multiplex CARS microspectroscopy. CARS spectra of the trans-fatty acid-fed cells showed that they accumulated cis- and trans-type lipids. Time-lapse observations also showed that cis-fats degraded significantly faster than trans-fats.
The authors developed the elliptical spot-excitation multiplex CARS (coherent anti-Stokes Raman scattering) spectroscopy microscope capable of high-speed spectroscopic imaging and applied it to the observation of trans fat metabolism to observe fat lipolysis by spatial resolution at the cellular level.
Samples were 3T3L1 cells differentiated into adipose-like cells and fed with the elaidic acid. We measured fat lipolysis with isoproterenol supplemented medium by time-lapse multiplex CARS microspectroscopy. CARS spectra of the trans-fatty acid-fed cells showed that they accumulated cis- and trans-type lipids. Time-lapse observations also showed that cis-fats degraded significantly faster than trans-fats.