[20p-A305-1~23] 3.3 Biomedical optics (formerly 3.4)

1:30 PM - 1:45 PM JST(4:30 AM - 4:45 AM UTC)

[20p-A305-3] Evaluation of Viscoelastic Properties of Vitreous using Brillouin Spectroscopy towards Diagnosis of Ocular Diseases

〇Hidekazu Ishitobi^1,2,3, Shunsuke Tokumitsu², Yasushi Inouye^1,2,3 (1.FBS, Osaka Univ., 2.Applied Physics, Osaka Univ., 3.PhotoBIO-OIL, AIST-Osaka Univ.)

Keywords:

Brillouin scattering,Viscoelasticity,Vitreous

Vitreous comprises a fibrous meshwork of collagen, and the interstices of the meshwork contain hyaluronic acid (HA) and water retained by HA. Age-related deterioration of HA alters the viscoelasticity of the vitreous, leading ocular diseases such as retinal detachment and eye floaters. However, the viscoelasticity of the vitreous has not yet been reported to be examined quantitatively in a non-invasive manner. In this study, we have determined the viscoelasticity of porcine vitreous with a Brillouin spectroscopic system by employing hyaluronidase (HAase) that induces degradation of HA to mimic aging vitreous. An increase in elastic modulus was observed in HAase-injected vitreous compared to PBS-injected vitreous. The increase is considered to attribute to the aggregation of collagen fibers due to the degradation of HA in the vitreous.