[21p-P10-1~5] 3.1 Basic optics and frontier of optics (merged with formerly 3.2 Equipment optics and materials)

[21p-P10-3] Spherical Aberration Correction by Multiplexed Focal Spots

〇Yuichi Kozawa¹, Yuuki Uesugi¹, Shunichi Sato¹ (1.IMRAM, Tohoku Univ.)

Keywords:

laser scanning microscopy,spatial light modulator,computer generated hologram

In this study, we propose a method to evaluate and correct spherical aberration in laser scanning microscopy by observing the axial information of PSFs from a single image acquisition using multiplexed focal spots produced by wavefront control of an excitation light beam. This method enables the prompt preparation of a dark spot applicable to STED microscopy even under the presence of spherical aberration in high NA focusing conditions.