Presentation Information

[22a-P04-2]Mechanical stimulation of cardiomyocyte aggregates with different phases and amplitudes using real-time feedback mechanism

〇(B)Kyotaro Kanazashi1, Ayu Sasaki1, Kaito Kojima1, Arata Nagai1, Hiromu Kuwabara1, Yuuta Moriyama1,2, Toshiyuki Mitsui1 (1.Aogaku Univ., 2.PRESTO, JST)

Keywords:

cardiomyocyte,Real-Time Feedback,biophysics

Mechanical stimulation affects the heart. Therefore, we developed a device that can control the phase of stimulation using feedback the autonomous beating of cardiomyocytes in real time and investigated the effects of mechanical stimulation on cardiomyocyte assemblies. One-dimensional cardiomyocyte assemblies were created using HH26 chicken hearts. In this presentation, we will focus on the sensitivity to external stimuli, examining how it varies based on both the amplitude and timing of the stimuli. We will delve into the transient changes in autonomous beating that these stimuli induce and explore the subsequent recovery processes.