[23p-12C-1~13] 3.3 Biomedical optics (formerly 3.4)

1:45 PM - 2:00 PM JST(4:45 AM - 5:00 AM UTC)Presentation by Applicant for JSAP Young Scientists Presentation Award

[23p-12C-2] Molecular Analysis of Intracellular Lipid Droplets by Using Raman Spectroscopy for metabolic dysfunction-associated steatotic liver disease (MASLD)

〇Tsuyoshi Takashina¹, Kazuki Yasumaru¹, Katsuya Sato², Takeshi Yasui³, Takeo Minamikawa^3,4 (1.Grad. Sch. Sci. Tech. Innov. Sci. Tokushima Univ., 2.Grad. Sch. Tech. Ind. Soc. Sci., Tokushima Univ., 3.pLED, Tokushima Univ., 4.Grad. Sch. Engg. Sci., Osaka Univ.)

Keywords:

raman spectroscopy,MASLD,lipid droplets

A more detailed understanding of the mechanisms of lipid uptake and accumulation in hepatocytes is important to elucidate the disease mechanisms of MASLD and to achieve a very early diagnosis at a reversible and treatable stage. In this study, we investigated the pathological role of lipid accumulation in hepatocytes by Raman analysis of HepG2 cells cultured in a mixed fatty acid/cholesterol culture medium. We found that the degree of unsaturation, an index to assess lipid uptake, decreased with increasing cholesterol concentration in the culture medium.