Presentation Information

12:15 PM - 12:30 PM JST(3:15 AM - 3:30 AM UTC)Presentation by Applicant for JSAP Young Scientists Presentation Award

[24a-1BN-12]Evaluation of bacterial activity based on electrochemical properties of formazan dye

〇Akira Tokonami¹, Hikaru Ikeda¹, Miyu Tanabe², Yasuhiro Sadanaga^1,2, Hiroshi Shiigi^1,2 (1.Osaka Metropolitan Univ., 2.Osaka Prefecture Univ.)

Keywords:

Cell activity,Live bacteria

MTT, a type of tetrazolium salt, permeates cell membranes and is reduced to insoluble MTT formazan by metabolism-related substances produced in living cells. In this study, we focused on the electrochemical response of MTT in bacterial suspensions and succeeded in estimating the number of viable cells from the magnitude of their redox current. The possibility of evaluating the enzyme activity of single cells was also discussed by spectroscopic analysis using microscopic observation.

Back to Session information