Presentation Information

9:15 AM - 9:30 AM JST(12:15 AM - 12:30 AM UTC)Presentation by Applicant for JSAP Young Scientists Presentation AwardEnglish Presentation

[24a-1BN-2]A New Delivery Technique for Analytes into Narrow Hotspots for Ultra-sensitive SERS at Single-protein Level

〇(DC)Tianxu Gao¹, Hideyuki Mitomo², Xu Shi³, Yusuke Yonamine², Kuniharu Ijiro² (1.Grad. Sch. Life Sci., Hokkaido Univ., 2.RIES, Hokkaido Univ., 3.CRIS, Hokkaido Univ.)

Keywords:

Surface-enhanced Raman Scattering,Single-molecule Detection,Molecular Delivery to Hotspots

The surface-enhanced Raman scattering (SERS) is a powerful tool in label-free and sensitive analyte detection, with the localized-enhanced optical near field (hotspot) in a narrow gap area. However, general SERS substrates are unsuitable for detecting proteins whose large volume hinders their approach to tiny hotspots. Herein, we design a novel strategy to deliver proteins into hotspots specifically on a structurally tunable hydrogel-based SERS substrate. The substrate is fabricated by a triangular-shaped gold nanoplate array and a thermo-responsive hydrogel. This strategy shows an ultra-sensitivity for protein detection at the single-molecule level.

Back to Session information