講演情報
[24a-1BN-2]A New Delivery Technique for Analytes into Narrow Hotspots for Ultra-sensitive SERS at Single-protein Level
〇(DC)Tianxu Gao1, Hideyuki Mitomo2, Xu Shi3, Yusuke Yonamine2, Kuniharu Ijiro2 (1.Grad. Sch. Life Sci., Hokkaido Univ., 2.RIES, Hokkaido Univ., 3.CRIS, Hokkaido Univ.)
キーワード：
Surface-enhanced Raman Scattering、Single-molecule Detection、Molecular Delivery to Hotspots
The surface-enhanced Raman scattering (SERS) is a powerful tool in label-free and sensitive analyte detection, with the localized-enhanced optical near field (hotspot) in a narrow gap area. However, general SERS substrates are unsuitable for detecting proteins whose large volume hinders their approach to tiny hotspots. Herein, we design a novel strategy to deliver proteins into hotspots specifically on a structurally tunable hydrogel-based SERS substrate. The substrate is fabricated by a triangular-shaped gold nanoplate array and a thermo-responsive hydrogel. This strategy shows an ultra-sensitivity for protein detection at the single-molecule level.