[25p-12B-1~13] 3.7 Optical measurement, instrumentation, and sensor (formerly 3.8)

3:15 PM - 3:30 PM JST(6:15 AM - 6:30 AM UTC)

[25p-12B-7] Development of High-resolution Light-field Microscopy and Application to Measurement of Biological Functions

〇Ryuki Imamura¹, Naoya Kataoka², Shin Usuki³, Takuma Sugi¹ (1.Hiroshima Univ., 2.Nagoya Univ., 3.Shizuoka Univ.)

Keywords:

bioimaging,imaging

Light-field microscopy (LFM) enables ‘scan-less’ acquisition of 3D volumetric image by postprocessing single 2D camera snapshots recorded via a microlens array. Temporal resolution is 100-fold faster than that of confocal microscopy because LFM can capture 3D in a single camera frame. Yet, its spatial resolution is 10-fold lower. Here we develop high-resolution LFM with submicron XY resolution and 1.5 um Z resolution. This high-resolution light-field microscopy will enable 3D volumetric imaging 100-fold faster than confocal microscopy in freely behaving C. elegans and Mouse slices.