[10a-N305-1~11] CS.11 Code-sharing session of 12.6&12.7

10:45 AM - 11:00 AM JST(1:45 AM - 2:00 AM UTC)Presentation by Applicant for JSAP Young Scientists Presentation Award

[10a-N305-7] Investigation of Gene Detection for Multiplex test of Bloodstream Infection

〇Saaya Shimizu¹, Yoshimitsu Yanagawa¹, Kenichi Takeda¹, Tomoyuki Sakai¹ (1.Hitachi, Ltd.)

Keywords:

microfluidics,bacterial test,gene-based test

To achieve bacterial test for bloodstream infection within one hour, we are developing microfluidic device-based gene testing system. Here, in our testing flow, we report about DNA detection using microfluidic microarray. To achieve multiplex test, we investigated target DNA labeling methods that enables rapid DNA detection under 40-plex PCR primers. We selected a labeling method using fluorescent Streptavidin (SA) which could reduce background noise. Using fluorescent SA method, we achieved target DNA detection in 4.5 minutes.