[10a-N403-1~10] 7.1 X-ray technologies

11:00 AM - 11:15 AM JST(2:00 AM - 2:15 AM UTC)

[10a-N403-7] Development of a cellular sugar concentration sensor by combining slow freezing method with cryo-micro-X-ray CT

〇Akio Yoneyama^1,2, Masahide Kawamoto¹, Kazuhiko Shiranita¹, Midori Yasuda³, Rika Baba², Tatsuhiko Kodama⁴, Satoshi Takeya⁵ (1.SAGA LS, 2.Hitachi, 3.Nishi-Kyushu Univ., 4.Saga Fruit Tree Experiment Station, 5.AIST)

Keywords:

micro X-ray CT,slow freezing,sugar sensor

We have developed a novel “cell sugar sensor” that enables the detection of sugar content within individual plant cells by combining slow freezing and cryo-micro X-ray computed tomography (CT). This sensor utilizes the fact that the patterns formed by sugars precipitated through slow freezing are correlated with sugar content. Using the SAGA LS BL07 cryo-micro X-ray CT system, we conducted proof-of-concept experiments on pears with varying sugar contents. The results showed that the sensor can detect sugar content at the single-cell level.