[10p-N104-1~10] 3.3 Biomedical optics

2:00 PM - 2:15 PM JST(5:00 AM - 5:15 AM UTC)Presentation by Applicant for JSAP Young Scientists Presentation Award

[10p-N104-3] Evaluation of cellular structures in cells cultured and rapidly frozen on coverslips: Toward time-deterministic correlative light and electron microscopy

〇Yuki Maeda¹, Kosuke Tsuji¹, Chieko Saito², Kaho Fujimori², Masahito Yamanaka¹, Masahide Kikkawa², Katsumasa Fujita^1,3 (1.UOsaka, 2.UTokyo, 3.UOsaka OTRI)

Keywords:

electron microscopy,cryo-optical microscopy,correlative light and electron microscopy

Time-deterministic cryo-optical microscopy, which we have developed, provides spatiotemporal information on cellular dynamics up to the moment of cryofixation by enabling fixation during optical microscopic observation. By combining time-deterministic optical and electron microscopy, it becomes possible to implement a time-deterministic CLEM, which allows analysis that correlates spatiotemporal information on intracellular dynamics with ultrastructural details. In this study, we evaluated the ultrastructure of cells cryofixed using a method similar to that of time-deterministic cryo-optical microscopy, in order to assess its applicability to time-deterministic CLEM.